The main difference between a road bike wheel with a 26-inch diameter and one with a 700c diameter lies in their sizing standards and typical applications.
26-inch wheels are commonly used on mountain bikes and some hybrid bikes. They have a rim diameter of 559 millimeters (mm). These wheels are designed to accommodate wider tires, typically ranging from 1.5 to 2.3 inches in width. The 26-inch size has been popular for many years, especially in the mountain biking community, but it has become less common in recent years as larger sizes gained popularity.
On the other hand, 700c wheels are the standard size for most modern road bikes, as well as many cyclocross and gravel bikes. The 700c designation refers to the approximate outer diameter of the tire, which is 700 millimeters. The actual rim diameter is 622 mm. These wheels are designed to accommodate narrower tires commonly used in road cycling, typically ranging from 23 to 32 mm in width. The larger diameter of 700c wheels allows for faster rolling and smoother performance on paved roads.
It's important to note that the difference in wheel size affects more than just the diameter. The overall geometry and frame design of a bike are often optimized for a specific wheel size, so you cannot easily interchange 26-inch and 700c wheels without also considering frame compatibility, brake compatibility, tire clearance, and other factors.